AN APPLICATION OF CONTROL REGION SEQUENCE AS A MATRILINEAGE MARKER FOR ELLIOT'S PHEASANT OF A ZOO POPULATION

ABSTRACT

Control region sequence, an mtDNA marker, was usually used in phylogenesis analysis in species level or genetic structure study among populations. In this study, enlightened by its character of maternal heredity in vertebrates, we used control region sequence as a matrilineage marker for Elliot's pheasant (Syrmaticus ellioti) of Ningbo Zoo population. In Ningbo Zoo, 36 individuals of Elliot's pheasant were descendants from three female founders introduced in 1988. Three control region haplotypes (Ha, Hb, Hc) were identified by six variable nucleotide positions among the control region sequences over 36 individuals. The number of haplotypes was accorded with the number of female founders. Total 20 individuals (C04, C06, C08–11, C14, C20, C21, C23–29, C32, C34–36) shared haplotype a, while 12 individuals (C01, C05, C07, C12, C13, C16–19, C22, C30, C33) shared haplotype b and 4 individuals (C02, C03, C15, C31) shared haplotype c. Those individuals sharing the same haplotype were offspring from one female founder. In other words, there were three maternal lineages and the simple relationship among individuals was indicated. As a result, it seemed that the control region sequence was a useful marker for identification of matrilineage in this study. Meanwhile, the matrilineage information may be compensatory data if there were no any pedigree records in captive species for breeding management.

Keywords:

• Control region sequence
• Elliot's pheasant
• Genealogy
• Matrilineage
• Haplotype

ACKNOWLEDGMENTS

We would like to thank Ningbo Zoo for providing all the samples. The study is supported by grants from the National Nature Science Foundation of China (no. 30330050 and no. 30470232).

Notes

*Number (vertical) showing the variation positions among haplotypes.