Abstract
Four basic drugs were separated by capillary electrophoresis(CE) using cyclodextrins(CDs) including β-CD, hydroxypropyl-β-CD(HP-β-CD), heptakis-2,6-di-O-methyl-β-CD(DM-β-CD) and carboxymethyl-β-CD (CM-β-CD) as chiral selectors. The effects of CM-β-CD concentration, pH of buffer solution, applied voltage and operation temperature were investigated. Under the conditions of 100 mmol/L H3PO4-triethanolamine buffer at pH 3.1 containing various amounts of CM-β-CD, baseline separation of the enantiomers of salbutamol, norephedrine, ofloxacin and atenolol could be achieved.
ACKNOWLEDGMENTS
This work was financially supported by the Natural Science Foundation of Zhejiang Province. We thank Prof. S. Zeng for providing the racemic basic drugs.