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Analytical Letters Volume 34, 2001 - Issue 6
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Original Articles

DIRECT DETERMINATION OF NICOTINE IN ANTISMOKING PHARMACEUTICAL PRODUCTS AND IN TOBACCO USING AN INHIBITION BIOSENSOR

Luigi Campanella Department of Chemistry, University of Rome ‘La Sapienza’ P.le Aldo Moro, Roma, 5 - 00185, Italy
,
Gabriele Favero Department of Chemistry, University of Rome ‘La Sapienza’ P.le Aldo Moro, Roma, 5 - 00185, Italy
&
Mauro Tomassetti Department of Chemistry, University of Rome ‘La Sapienza’ P.le Aldo Moro, Roma, 5 - 00185, Italy
Pages 855-866 | Received 01 Dec 2000, Accepted 01 Feb 2001, Published online: 23 Aug 2006
 
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Abstract

A bienzymatic inhibition biosensor, consisting of a hydrogen peroxide amperometric electrode coupled to a functionalised nylon membrane chemically bonding the enzymes butyrylcholinesterase and choline oxidase and using butyrylcholine as substrate, was prepared and tested for the analysis of nicotine. The system was applied to the analysis of several pharmaceutical formulations containing nicotine used to treat addiction and to determine the nicotine content of different types of commercial tobacco.

ACKNOWLEDGMENT

Work carried out with the financial support of Consiglio Nazionale delle Ricerche (CNR) of Italy, Target Project MADESS and Consorzio Interuniversitario Nazionale ‘La Chimica per l'Ambiente’ (INCA).

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