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Analytical Letters Volume 35, 2002 - Issue 12
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Original Articles

CAPACITIVE IMMUNOSENSOR FOR THE DETERMINATION OF SCHISTOSOMA JAPONICUM ANTIGEN

Ya-Min Zhou State Key Laboratory for Chemical/Biosensing Technology and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China
,
Shen-Qin Hu State Key Laboratory for Chemical/Biosensing Technology and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China
,
Zhi-Xiang Cao State Key Laboratory for Chemical/Biosensing Technology and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China
,
Guo-Li Shen State Key Laboratory for Chemical/Biosensing Technology and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. ChinaCorrespondence[email protected]
&
Ru-Qin Yu State Key Laboratory for Chemical/Biosensing Technology and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China
Pages 1919-1930 | Received 24 May 2002, Accepted 29 May 2002, Published online: 02 Feb 2007
 
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ABSTRACT

A capacitive immunosensor, based on Schistosoma japonicum antibody (SjAb) immobilized on the gold electrode surface via protein A (PrA), has been prepared for the detection of Schistosoma japonicum antigen (SjAg). The assay conditions were optimized, including the amount of SjAb loading at the electrode, the ionic strength and pH of the solution. The optimized system can measure SjAg in the range of 0.4–18 ng/mL with a detection limit of 0.1 ng/mL. It exhibits some advantages such as simplicity of fabrication, rapidity of assay, and good sensitivity.

ACKNOWLEDGMENT

This work was supported by the National Natural Science Foundation of China (Grants No. 29975006, 20075006), the Foundation for Ph.D. Thesis Research (No. 20010532008), and the Foundation of Science Commission of Hunan Province.

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