Abstract
Peroxisomicine A1 (PA1) is a natural product that shows biological activity. After its extraction and isolation from the plant, there is still between 3 and 5% remaining of an isomer (isoperoxisomicine A1, isoPA1), which is also present in the original matrix. A simple and reliable quantification method has been developed by means of proton nuclear magnetic resonance spectroscopy to determine the isoperoxisomicine amount in these PA1 batches. Two methods were applied to the quantification: the relative area method and an absolute method by using an internal standard. No significant differences were found between them. The simplicity of the first one makes it desirable for routine analysis. The validated method has adequate linearity and precision, because it allows measuring as low as 1% of isoPA1 content.
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Acknowledgments
We wish to thank CONACYT for financial support in the form of a fellowship for A.F.R. and grant number 26497‐N, as well as PAICYT for financial support through grant CA‐375‐00.