Lipoprotein Isolation on Cellulose Acetate Membrane for Cholesterol Estimation from Blood Samples of Cardiac Patients

Abstract

The lipoproteins have been successfully isolated electrophoretically from the blood samples of patients having suffered from type III, hyperlipoproteinemia but survived myocardial infarction. The low‐density lipoprotein (LDL) and high‐density lipoprotein (HDL) fraction from the cellulose acetate membrane is cut, and the total cholesterol and cholesterol of the LDL and HDL are extracted. The cholesterol is determined using an immobilized cholesterol esterase/oxidase column in a flow system.

Keywords:

• Cellulose acetate membrane
• Myocardial infarction
• Hyperlipoproteinemia
• Cholesterol
• Electrophoresis

Acknowledgments

The authors are thankful to the International Biotechnology Consortium, Italy, and the Institute of Biochemistry, University of Balochistan, Quetta, for provision of necessary funds and partial lab facility. The laboratory help of Prof. Stefano Girotti of the University of Bologna is also greatly acknowledged. The writing help and guidance of M. Masoom Yasinzai, Professor, Institute of Biochemistry, University of Balochistan, Quetta, and heart physician, Dr. M. Hashim Khan, Professor of Cardiology Department, Government Sandeman Civil Hospital, Quetta, were valuable throughout the study.