VASOCONSTRICTIVE RESPONSE OF RAT MESENTERIC ARTERIOLES FOLLOWING INFUSION OF CROSS-LINKED, POLYMERIZED, AND CONJUGATED HEMOGLOBIN SOLUTIONS

Figures & data

Table 1. Physicochemical Parameters of Hemoglobin-Based Oxygen Carriers and HES 200

	Hb-Dex-BTC	αα-Hb	O-raffinose-Hb	HES 200
Concentration (g/dL)	8.5	8.2	10.0	6.0
Free Hb (%)	4.9	< 5	4.2	NA
Average molecular (daltons)	160,000	64,000	120,000	MWW: 200,000
				MWN: 60,000
P50 (Torr)	23	29	34	NA
Viscosity^a (cSt)	2.11	0.97	1.24	1.93

P₅₀: oxygen half-saturation pressure of Hb measured at 37°C; MW_W: weighted-average molecular weight; MW_N: number-averaged molecular weight; NA: non applicable.

^aKinematic viscosity determined at 37°C with an automatic viscosimeter (Viscomatic VCD, Amtec, France).

Figure 1. A View of a rat mesenteric arteriole in basal conditions. c.t.: connective tissue; e.: endothelium; v.l.: vascular lumen. B) View of a digitized image of a mesenteric arteriole during the early step of determination of the diameter with Morphostar software. The arteriolar lumen is colored in red and the cursors used to calculate the arteriolar diameter are aligned with the vessel edges. C) View of 30 digitized images of a mesenteric arteriole during the last step of calculation of the diameter. Examples of the results of calculation are given in the table in the lefthand corner of the picture.

Figure 2. Variations of the inner diameter of rat mesenteric arterioles following intravenous infusion of HES 200 (n = 3), Hb-Dex-BTC (n = 3), αα-Hb (n = 4), or o-raffinose-Hb (n = 3). #: P < 0.05, postinfusion vs. preinfusion; ^*: P < 0.05, solution vs. HES 200; †: P < 0.05, solution vs. Hb-Dex-BTC; ‡: P < 0.05, solution vs. o-raffinose-Hb.