SEVENTY-TWO HOURS HYPOTHERMIC INTESTINAL PRESERVATION STUDY USING A NEW PERFLUOROCARBON EMULSION

Figures & data

Table I. Histological Score Used to Describe the Morphological Lesions of the Intestinal Graft After Preservation

Intestinal Histology Score
Villus Score	Crypt Score
0 Complete destruction	0 Complete destruction
1 Separation of villus epithelium from lamina propria, complete Villus sloughing (base of villi)	1 Separation of crypt cells from basal membrane, complete
2 Separation of villus epithelium from lamina propria, exceeding half of length of villi, villous sloughing moderate	2 Separation of crypt cells from basal membrane, moderate Persistance of intercellular adhesions
3 Separation of villus epithelium from lamina propria, apical	3 Separation of crypt cells from basal membrane, loss of intercellular adhesions
4 Normal	4 Normal

Table II. Evolution of Histological Score (0–8) During 72 h Preservation at +4°C Results Are Expressed as Mean ± SEM; n = 5 in Each Group; for Each Time Point, 5 Histological Samples Were Blindly Assessed

Preservation Duration	UW/UW Group 1	UW/FC Group 2	FU/FU Group 3	FU/FU +O2 Group 4
0h	7.5 ± 0.0	7.5 ± 0.0	8.0 ± 0.0	7.5 ± 0.0
12h	5.8 ± 0.3	5.3 ± 0.3	6.2 ± 0.1	7.1 ± 0.2
24h	4.4 ± 0.4	5.8 ± 0.1	5.8 ± 0.4	6.4 ± 0.2
48h	3.8 ± 0.8	4.4 ± 1.1	2.9 ± 0.5	4.9 ± 0.5
72h	2.4 ± 0.6	2.6 ± 1.1	3.3 ± 0.4	1.3 ± 0.3

Figure 1. Intestinal histological score after 24 h preservation: comparison between the 4 experimental groups. * p < 0.05 and ** p < 0.001versus group 1

group 1 (UW/UW)

group 2 (UW/FC)

group 3 (FU/FU)

group 4 (FU/FU+O₂)

Figure 2. Evolution of intestinal histological score: comparison of each experimental group with group 1 (UW/UW) (

). A. group 2 (

UW/FC) versus group 1 B. group 3 (

FU/FU) versus group 1 C. group 4 (

FU/FU+O₂) versus group 1 * p < 0.05 ** p < 0.001

Figure 3. Antioxidant status of intestinal tissue after 24 h preservation: comparison of groups 3 (

FU/FU) and 4 (

FU/FU+O₂) with group 1 (

UW/UW). −SH : total thiol functions GSH-P : glutathione peroxidase activity SOD : superoxide dismutase activity Cat : catalase activity * p < 0.05