SUPEROXIDE GENERATION FROM HUMAN POLYMORPHONUCLEAR LEUKOCYTES BY LIPOSOME-ENCAPSULATED HEMOGLOBIN

Figures & data

Table 1. Characteristics of NRC, Emply Liposomes, and Red Blood Cells (RBCs)

	Particle Size (μm)	Phospholipids (g/dL)	Hemoglobin (g/dL)	SOD Activity (kU/gHb)
NRC^*	0.25	3.4	6.2	23.5
Empty liposomes^*	0.12	3.3	0	0
RBC	7.8	ND	6.0 ± 0.2^†	24.8 ± 1.2^†

ND; not determined.

^* Data from TERUMO except for SOD activities.

^† Mean ± SD, n = 3.

Figure 1. Superoxide generation from human neutrophils by NRC and empty liposomes. Liposomes were added at a final concentration of 8% (v/v) to PMN suspension containing DMPO. Open circles, NRC; closed circles, the empty liposomes; closed triangles, the empty liposomes + 50 mU of SOD.

Figure 2. NRC-dose dependence of superoxide generation from human neutrophils. Liposomes were added to PMN suspension containing DMPO at the following concentrations; open symbols, NRC (circles, 10% (v/v); squares, 1%; triangles, 0.1%; diamonds, 0.01%); closed circles, 10% empty liposomes.

Figure 3. Effect of NRC and the empty liposomes on PMA-induced superoxide generation from human neutrophils. The liposomes were added at a final concentration of 10% (v/v) to PMN suspension containing DMPO, and then PMA was added to the reaction mixture. Squares, NRC; triangles, the empty liposomes; circles, PMA only.