CROSS-LINKED HEMOGLOBIN AS A POTENTIAL MEMBRANE FOR AN ARTIFICIAL RED BLOOD CELL

Figures & data

Figure 1. Benzoic acid in the side-by-side diffusion cell through the 5, 10, and 14 μm polycarbonate supports. (n=3) Error bars represent standard deviation.

Reaction 1. Chemical reaction of hemoglobin with the NHS-ester cross-linker.

Figure 2. Methemoglobin diffusion through the 4.65 mM DSS cross-linked hemoglobin membrane. Error bars represent standard deviation. (n=3)

Table 1. Summary of Methemoglobin and Benzoic Acid Diffusion Through the Hemoglobin Membrane Cross-Linked with 4.65 mM DSS in o-Xylene (n=3)

	Methemoglobin Diffusion		Benzoic Acid Diffusion
Cross-Linking Time (min)	(DA/h)eff (cm^3/sec)	% Reduction	(A/h)eff (cm)	% Reduction
60	0.044 ± 0.01	94.4	—	—
80	0.027 ± 0.006	96.6	—	—
100	0.0061 ± 0.002	99.2	—	—
120	0.0058 ± 0.001	99.3	33.3 ± 3.7	81.1
140	0.0044 ± 0.0002	99.4	22.2 ± 0.8	87.4

Figure 3. Methemoglobin diffusion through the hemoglobin cross-linked membrane, cross-linked with saturated DST. Error bars represent standard deviation. (n=3)

Table 2. Summary of Methemoglobin and Benzoic Acid Diffusion Through the Hemoglobin Cross-Linked Membrane Cross-Linked with Both 4.65 and 0.46 mM DSG in o-Xylene (n=3)

	Methemoglobin Diffusion		Benzoic Acid Diffusion
Cross-Linking Time (min)	(DA/h)eff (cm^3/sec)	% Reduction	(A/h)eff (cm)	% Reduction
4.65 mM DSG
5	0.0018 ± 0.0007	99.8	30.5 ± 0.9	82.7
15	0.0019 ± 0.00004	99.8	—	—
20	0	100	—	—
30	0	100	19.8 ± 2.1	88.7
60	0	100	—	—
0.46 mM DSG			—	—
20	0.05 ± 0.002	93.7	37.1 ± 1.5	78.9
40	0.0042 ± 0.001	99.5	—	—
60	0	100	22.6 ± 1.4	87.2