PLURONIC F-68 ENHANCED SHOOT REGENERATION IN A POTENTIALLY NOVEL CITRUS ROOTSTOCK

Figures & data

Table 1. Mean Increase in Biomass (g.f.wt.), Mean Percentage of Shoot/Buds Regenerated, and Mean Number of Shoots/Buds Regenerated per Explant for Epicotyl and Cotyledon Explants of Citrus depressa Cultured for 56 Days in Semi-solidified Medium Alone (Control) or Medium Supplemented with 0.5% (w/v) Pluronic F-68

	Explant Source
	Epicotyl	Cotyledon
Increase in biomass (mean g.f.wt.)
Control	2.5 ± 0.1	2.3 ± 0.1
Pluronic (0.5% w/v)	4.0 ± 0.2^*	2.4 ± 0.1
Proportion of explants exhibiting shoot/bud regeneration (mean %)
Control	71 ± 1	34 ± 4
Pluronic (0.5% w/v)	89 ± 3^*	65 ± 5^*
Mean number of shoot/buds per explant
Control	4.5 ± 0.2	1.1 ± 0.1
Pluronic (0.5% w/v)	8.3 ± 0.3^*	4.7 ± 0.3^*

Values are mean ± s.e.m., n = 3 throughout.

^* P < 0.05 compared to control [0% (w/v) Pluronic F-68] mean values.