STUDY OF INTERACTION OF MANNAN-BSA NEOGLYCOCONJUGATES WITH CONCANAVALIN A

Figures & data

Table 1. Characteristics of Used Mannans and Mannan-BSA Conjugates

Conjugate Sample	Aldehyde Content per Mannan [mol/mol]	Mw of Mannan [kDa]	React. Ratio Mann./BSA [mg/mg]	Mw of Conjug. [kDa]	Saccharide in Conjugate [wt. %]	Aprox. Ratio Mann./BSA in Conjg. [mol/mol]
CM1a	11	44.5	1:1	176	25	1:2
CM1b	11	44.5	1.5:1	144	34	1:1
CM1c	11	44.5	4:1	237	65	3:1
CM2a	20	42.0	1:1	246	28	1.5:2.5
CM2b	20	42.0	1.5:1	244	32	1:1
CM2c	20	42.0	4:1	256	58	2:1
CM3a	28	40.0	1:1	169	30	1:1.5
CM3b	28	40.0	1.5:1	156	29	1:1
CM3c	28	40.0	4:1	188	47	1.6:1

Figure 1. Precipitation profiles of mannan-BSA conjugates (with high content of mannan) with Con A. Panel A – dependence of mannan content (original or in conjugate) in precipitate on initial content of mannan (original or in conjugate) in the test. Panel B – dependence of Con A content in precipitate on initial content of mannan (original) or in conjugate) in the test. -•- original mannan, -○- CM1c, -□ - CM2c, -▵- CM3c.

Figure 2. Dependence of molar ratio mannan/Con A in precipitate on initial content of mannan-BSA conjugate in the test. Panel A – of conjugates prepared from M1-mannan. Panel B – of conjugates prepared from M2-mannan. Panel C – of conjugates prepared from M3-mannan. -•- CM1a – CM3a, -▪- CM1b–CM3b, -▵- CM1c–CM3c.

Figure 3. Dependence of molar ratio BSA/Con A in precipitate on initial content of mannan-BSA conjugate in the test. Panel A – of conjugates prepared from M1-mannan. Panel B – of conjugates prepared from M2-mannan. Panel C – of conjugates prepared from M3-mannan. -•- CM1a–CM3a, -▪- CM1b–CM3b, -▵- CM1c–CM3c.