VIRUS INACTIVATION IN HEMOGLOBIN SOLUTION BY HEAT TREATMENT

Figures & data

Table 1. VSV Infectivity Before and After the Heat Treatment

	VSV titer (log TCID50)
Atmosphere	Before	After	Log inactivation (log10)
Air	5.3 ± 0.21	−0.5^*	>5.8
CO	5.5 ± 0.26	−0.5^*	>6.0

Values are represented as mean ± SD (n = 3).

^*Below the detection limit.

Figure 1. Heat treatment of hemoglobin solution. Hemolysate was heated at 60°C for 1 hr under an air (stippled bar, n =3, mean ± SD) or CO (open bar, n = 5, mean ± SD) atmosphere. Methemoglobin rate (A), hemoglobin concentration (B) and protein concentration (C) in SFHs before and after the treatment.

Figure 2. SDS-PAGE and IEF of SFHs. Before (B) and after (A) the heat treatment under the air or CO atmosphere. A0, hemoglobin A₀; A₂, hemoglobin A₂. M.W., molecular weight marker.

Figure 3. Hemoglobin behavior before and after the heat treatment. Hemolysate was heated at 60°C for 1 hr under the air or CO atmosphere. Hemoglobin concentration (A), solution volume (B) and total hemoglobin amount (C) for SFH (open bar) and stroma (stippled bar). n = 3, mean ± SD.