Figures & data
Figure 1. Effects of the PEG-HbV on the fMLP-triggered chemotactic activity of the PMNs. The PMNs were incubated for 30 min at 37°C with various concentrations of PEG-HbV. Then, the cells were allowed to migrate for 30 min at 37°C. Values are means ± SE of four donors.
Figure 2. Effects of the PEG-HbV pretreatment on the CD11b (Mac-1) expression on the PMNs. The PMNs were pretreated with PEG-HbV at 60 mg/dl of Hb for 30 min at 37°C. The PMNs were then incubated with 1 μM fMLP or vehicle. The constitutive and fMLP-induced CD11b (Mac-1) expression was analyzed by flow cytometry. Data are representative of two separate experiments.
Figure 3. Effects of the PEG-HbV pretreatment on the gelatinase B (MMP-9) release from the PMNs. The PMNs were preincubated for 30 min with (circle) or without (square) PEG-HbV at 600 mg/dl Hb. After being washed, the PMNs were stimulated with 1 μM fMLP for the indicated times. The levels of gelatinase B released into supernatant were determined by a total MMP-9 ELISA system. Values are means ± SE (N=5).
Figure 4. Effects of the PEG-HbV pretreatment on the superoxide production in the PMNs. The PMNs were preincubated for 30 min with (hatched column) or without (open column) PEG-HbV at 600 mg/dl Hb. After the PMNs were washed, the fMLP (1 μM)-triggered superoxide production was measured by the cypridina luciferin analog-dependent chemiluminescence. Data represent the mean ± SE (N=9).
