IN VITRO STUDY OF A NOVEL LOW-DENSITY LIPOPROTEIN ADSORBENT

Figures & data

Table 1. Amount of Ligand Attached on Carrier vs. Its Adsorption Capacity (The Initial Concentrations of TC, TG, LDL-C, and HDL-C Were 248.6, 167, 183, and 57 mg/dl, Respectively)

Amount of Ligand on Carrier (mg/ml gel)	TC Amount Adsorbed (mg) (%)	TG Amount Adsorbed (mg) (%)	LDL-C Amount Adsorbed (mg) (%)	HDL-C Amount Adsorbed (mg) (%)
Control	0.275±0.06 (7.4±0.8)	0.231±0.11 (9.2±4.4)	0.222±0.01 (8.09±0.4)	0.041±0.01 (4.79±1.2)
1.68	0.956±0.17 (25.6±4.5)	0.576±0.07 (23±2.8)	0.921±0.16 (33.6±5.8)	0.106±0.01 (12.4±1.2)
3.20	1.131±0.14 (30.3±3.8)	0.651±0.15 (26±6.0)	1.066±0.04 (38.8±1.4)	0.126±0.04 (14.7±4.7)
4.08	1.413±0.29 (37.9±7.8)	0.826±0.14 (33±5.6)	1.281±0.23 (46.7±8.4)	0.158 ±0.03 (18.5±3.5)
6.42	1.849±0.23 (49.6±6.2)	0.927±0.34 (37±13.5)	1.562±0.19 (56.9±6.9)	0.163± 0.05 (19.1±5.8)
7.60	2.010±0.35 (53.9±9.4)	1.077±0.22 (43.0±8.8)	1.806±0.41 (65.8±14.9)	0.174±0.06 (20.3±7.0)

Figure 1. Adsorption amount vs. the amount of ligand. The initial concentrations of TC, TG, LDL-C, and HDL-C were 296.7, 167, 183, and 48 mg/dl, respectively.

Figure 2. Incubation time vs. adsorption percentage. The initial concentrations of TC, TG, LDL-C, and HDL-C were 296.7, 167, 183, and 48 mg/dl, respectively.

Table 2. Amount of Ligand Attached vs. Nonspecific Adsorption of Adsorbent

Amount of Ligand (mg/ml gel)	Albumin(g/l)		Total Immunoglobulin (g/l)
	Before	After	Before	After
Control	29.78±2.47	28.85±2.63	48.57±3.53	46.93±5.67
1.68	29.78±0.75	26.58±2.14	48.57±6.51	50.62±2.39
3.6	29.78±1.02	29.89±1.34	48.57 ±4.13	48.61±5.86
4.64	29.78±3.01	29.18±2.68	48.57±3.91	46.37±1.83
5.4	29.78±2.54	27.29±3.61	48.57±7.37	44.56±2.49
6.4	29.78±2.38	28.64±1.92	48.57±1.52	47.13±6.21

Table 3. The Leakage of Ligand After Steam Sterilization

Sample No.	1	2	3	4	5	6
Amount of ligand attached (mg/ml)	1.68	3.6	4.64	5.4	6.4	7.6
Leakage amount (mg)	0	0	0.03	0	0.02	0.01
Leakage (%)	0	0	0.65	0	0.3	0.1

Figure 3. The difference in adsorption capacity of the adsorbent before and after steam sterilization. The initial concentrations of TC, TG, LDL-C, and HDL-C were 234.8, 123, 176, and 47.3 mg/dl, respectively.