THE ROLE OF DIFFERENT CHEMICAL MODIFICATIONS OF SUPEROXIDE DISMUTASE IN PREVENTING A PROLONGED MUSCULAR ISCHEMIA/REPERFUSION INJURY

Figures & data

Table 1. Percentages of the Sole Flux Variations in the Four Groups at the Beginning of the Reperfusion (Mean±SD, n=15)

Animal Groups	F% variation
Control	−82±19.6^*
SOD	−44.6±6.5°
MPEG–SOD	−59.1±8.9
PacM–SOD	−57.7±15.1

ANOVA F=17.01 p<0.005.

SOD: superoxide dismutase; mPEG–SOD: monomethoxypolyethylene-glycol-SOD; PacM–SOD: poly-(acryloilmorpholine)-SOD.

Scheffé's post hoc multiple comparison test: ^*Control vs. mPEG–SOD (p<0.001), SOD and PacM–SOD (p<0.0005); °SOD vs. mPEG–SOD (p<0.05).

Table 2. Histomorphologic Data of the Vital Limbs (Mean±SD)

Group	n.	Fiber Diameter (μm)	% Fibers with Central Located Nuclei
Normal limbs	10	75.6±4.8	2.6±0.9
Control	5	67.2±6.8	28.7±4.7
SOD	9	49.2±10.9^**	45.9±14.8^#
MPEG–SOD	13	43.1±6.8°	87.4±5.5°°
PacM–SOD	11	53.3±12.3^*	46.9±16.4^#

ANOVA F=22.91; p<0.0005; F=93.34; p<0.0005.

SOD: superoxide dismutase; mPEG–SOD: monomethoxypolyethylene-glycol-SOD; PacM–SOD: poly-(acryloilmorpholine)-SOD.

Scheffé's post hoc multiple comparison test:

- Fiber diameter: ^*PacM–SOD vs. Normal limbs (p<0.0005); °mPEG–SOD vs. Control and Normal limbs (p<0.0005); ^**SOD vs. Control (p<0.05) and Normal limbs (p<0.0005).

- % Fibers with central located nuclei: °°mPEG–SOD vs. Normal limbs, Control, SOD and PacM–SOD (p<0.0005), ^#SOD and PacM–SOD vs. Normal limbs (p<0.0005).