Immobilization of Glutathione-s-transferase Within Cross-Linked Gelatin Cylindrical Molds

Figures & data

Table 1. Effect of enzyme, gelatin and formaldehyde concentration on the immobilization efficiency of cytosolic GST preparation by means of activity yield

Cytosolic Protein (mg/ml gelatin)	Activity yield (%)	Gelatin (%)	Activity yield (%)	Formaldehyde (%)	Activity yield (%)
1	65	2.5	67.6	0.2	64
3	71.7	5	74.1	0.5	74.2
6	86.8	7.5	77.7	0.8	82.2
8	87.5	10	88.5	1.0	87.8
10	87.9	12.5	78.4	1.5	71.3

^*1 mg cytosolic protein = 0.4 Units, gelatin; 10%.

Figure 1. The effect of pH on the activity of free(▪) and immobilized(•) GST activity (sodium acetate; pH 3.0–6.0, phosphate; pH 6.5–7.5, borate; pH 8.0–9.0).

Figure 2. The effect of Temperature on the activity of free(▪) and immobilized(•) GST activity.

Figure 3. Thermal Stability of free(▪) and immobilized(•) GST.

Figure 4. Quantities of 2,4‐dinitrophenyl‐GSH in a stirred batch reaction of glutathione conjugation.

Figure 5. Conversion curve of CDNB in a batch reactor system (So→30 μmol).