Osteoblasts Cultured from Osteoporotic Bone: A Comparative Investigation on Human and Animal‐Derived Cells

Figures & data

Table 1. Cell Viability and Synthetic Activity of Primary Osteoblasts Derived from Osteopenic Human (hOB), Rat (rOB) and Sheep (sOB) Bone Without 1,25(OH)₂D₃ Stimulation (Mean ± SD, n = 10 Replicates)

Osteopenic cultures	MTT (OD 550 nm)	ALP (IU/l)	OC (ng/ml)	NO (µM)	PICP (ng/ml)	IL‐6 (pg/ml)	TGF‐β1 (pg/ml)
hOB	1.53 ± 0.03	41.5 ± 1.4^a	23.4 ± 1.5^b	3.58 ± 0.14^a	27.5 ± 0.7^a	185 ± 35^a	1066 ± 23^d
rOB	1.71 ± 0.20	12.8 ± 1.7	13.5 ± 1.3	2.48 ± 0.21	10.2 ± 0.5	32 ± 6	641 ± 125
sOB	1.97 ± 0.08	37.0 ± 1.1	22.2 ± 2.9^c	3.20 ± 0.14	17.4 ± 1.4	94 ± 4	442 ± 19

Kruskall‐Wallis Multiple Comparison test: ^a, hOB versus rOB (p < 0.01); ^b, hOB versus rOB (p < 0.05); ^c, sOB versus rOB (p < 0.05); ^d, hOB versus sOB (p < 0.01).

Table 2. Cell Viability and Synthetic Activity of Primary Osteoblasts Derived from Osteopenic Human (hOB), Rat (rOB) and Sheep (sOB) Bone with 1,25(OH)₂D₃ Stimulation (Mean ± SD, n = 10 Replicates)

Osteopenic cultures	MTT (OD 550 nm)	ALP (IU/l)	OC (ng/ml)	NO (µM)	PICP (ng/ml)	IL‐6 (pg/ml)	TGF‐β1 (pg/ml)
hOB	1.51 ± 0.03	68.3 ± 0.7^a	43.4 ± 2.3	4.08 ± 0.29^a	23.0 ± 0.9^a	182 ± 34^a	1267 ± 58^c
rOB	1.67 ± 0.13	36.3 ± 1.5	49.7 ± 2.9	2.54 ± 0.06	10.0 ± 0.7	27 ± 4	944 ± 176
sOB	1.95 ± 0.09	47.0 ± 1.6	36.0 ± 1.4^b	3.33 ± 0.10	14.0 ± 2.3	88 ± 2	454 ± 14

Kruskall‐Wallis Multiple Comparison test: ^a, hOB versus rOB (p < 0.01); ^b, sOB versus rOB (p < 0.01); ^c, hOB versus sOB (p < 0.01).

Table 3. Percentage Variations Between Vitamin 1,25(OH)₂D₃‐Stimulated and Unstimulated Primary Osteoblasts from Osteopenic Human (hOB), Rat (rOB) and Sheep (sOB) Bone

Parameters	Species			Kruskall‐Wallis
	Humans^1	Rats^2	Sheep^3
Osteopenic cultures
MTT	− 2.81 ± 0.78	− 4.09 ± 6.33^a	− 2.59 ± 5.02	ns
ALP	34.51 ± 15.44^a	41.98 ± 10.29	25.62 ± 5.62^a	ns
NO	12.40 ± 2.91^b	2.59 ± 0.29^a	4.06 ± 4.78	1 vs 2: p < 0.05
OC	66.36 ± 8.81^a	258.15 ± 39.83^a	57.45 ± 5.39^a	1,3 vs 2: p < 0.05
PICP	− 9.97 ± 6.24	− 7.95 ± 7.72^a	− 24.60 ± 7.69^c	ns
IL‐6	− 7.72 ± 2.95^a	− 17.13 ± 0.57^a	− 8.96 ± 2.93^a	1,3 vs 2: p < 0.05
TGF‐β1	23.58 ± 3.88 ^d	50.95 ± 38.15 ^c	2.82 ± 6.18 ^a	2 vs 3: p < 0.05
Normal cultures
MTT	− 2.66 ± 0.67	− 0.26 ± 5.23	− 2.91 ± 2.09	ns
ALP	49.85 ± 1.05	42.84 ± 11.91	34.25 ± 6.00	1 vs 3: p < 0.05
NO	15.87 ± 11.34	4.96 ± 0.94	3.28 ± 6.35	1 vs 2,3: p < 0.05
OC	97.8 ± 8.15	151.82 ± 10.00	60.24 ± 6.43	2 vs 3: p < 0.05
PICP	− 9.98 ± 1.85	− 13.76 ± 9.04	− 13.24 ± 5.91	ns
IL‐6	− 5.49 ± 3.39	− 19.29 ± 1.28	− 3.28 ± 2.28	1,3 vs 2: p < 0.05
TGF‐β1	21.06 ± 1.72	23.08 ± 3.64	24.47 ± 4.51	ns

Mann‐Whitney test between Osteopenic and Normal cultures: ^a, p < 0.0005; ^b, p < 0.001; ^c, p < 0.005; ^d, p < 0.05.