Studies on the Preparation of Chitosan Microcarriers Cross‐Linked by Oxidized Lactose and Culture of Primary Hepatocytes

Figures & data

Table 1. The Effect of the Amount of Oxidized Lactose on the Properties of Chitosan Microcarriers(CMs).

Amount of oxidized lactose used as pre‐cross‐linker(ml)	Amount of oxidized lactose supplemented (ml)	Water content (%)	Density (g/ml)	Surface and rigidity
6	10	95.4	1.02	Irregular surface, bad rigidity
6	20	93.8	1.03	Regular surface, good rigidity and elasticity
6	25	90.6	1.05	Regular surface, good rigidity and elasticity

^*chitosan concentration: 2.5%w/v.

Figure 1. SEM of rat hepatocytes on_CMs after three days culture (a) on CMs cross‐linked by glutaraldehyde (b) on CMs cross‐linked by oxidized lactose.

Figure 2. Albumin secretion of hepatocytes cultured on the CMs (a) Cytodex 3 (b) CMs cross‐linked by glutaraldehyde (c) CMs cross‐linked by oxidezed lactose.

Figure 3. Glucose metabolism of hepatocytes cultured on CMs (a) Cytodex 3 (b) CMs cross‐linked by glutaraldehyde (c) CMs cross‐linked by oxidized lactose.