Arginine Selective Biosensor Based on Arginase‐Urease Immobilized in Gelatin

Figures & data

Figure 1. The effect of pH on the biosensor response (Tris‐HCl; pH 7.0–8.5, borate; pH 9.0–9.5, arginine conc.; 0.25 mM, 25°C).

Figure 2. The effect of temperature on the biosensor response (Arginine conc.; 0.25 mM, 25°C).

Table 1. Arginine Detection in Blood Serum Samples

	Signal detected amount (mM)
	(ΔmV)	Arginine	Urea
Arginine standard soln (0.0625 mM)	2	0.069
Blood serum (0.25 ml)	2	0.069
Arginine standard soln (0.0625 mM) in serum	4	0.188
Urea standard soln (0.0625 mM)	1.5**	0.058**
Blood serum (0.25 ml)	0**	0**
Urea standard soln (0.0625 mM) in serum	1.5**	0.058**

^*Obtained by arginase‐urease system.

^**Obtained by urease system.