Encapsulation of Enzymes in Liposomes: High Encapsulation Efficiency and Control of Substrate Permeability

Figures & data

Figure 1. Effect of lipid concentration on AChE encapsulation.

Figure 2. Effect of freeze–thaw number on AChE encapsulation.

Figure 3. (A) Effect of filter composition on AChE encapsulation percentage: encapsulated enzymatic activity/total activity. (B) Enzyme recovery percentage: Total enzymatic activity after extrusion/total enzymatic activity. The filter used: Cellulose Acetate (first column) and Nylon (second column).

Figure 4. Specific activity (v/[Et]) of acetylcholinesterase as a function of acetylthiocholine concentration outside the liposome. Three enzyme formulations were tested, free AChE in absence of liposome, encapsulated AChE in liposomes with or without the porin OmpF.