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Original

Acetylcholinesterase (AChE) Electrodes Based on Gelatin and Chitosan Matrices for the Pesticide Detection

&
Pages 427-442 | Published online: 11 Jul 2009

Figures & data

Figure 1. (a) Optimum pH of gelatin based AChE electrode (pH 7.0–8.2; potassium phosphate buffer (♦), pH 8.0–9.0; borate buffer (•). (b) Optimum pH of chitosan based AChE electrode. (pH 7.0–8.2; potassium phosphate buffer (♦), pH 8.0–9.5; borate buffer (•).

Figure 1. (a) Optimum pH of gelatin based AChE electrode (pH 7.0–8.2; potassium phosphate buffer (♦), pH 8.0–9.0; borate buffer (•). (b) Optimum pH of chitosan based AChE electrode. (pH 7.0–8.2; potassium phosphate buffer (♦), pH 8.0–9.5; borate buffer (•).

Figure 2. Optimum temperature of gelatin (•) and chitosan (▪) based AChE electrode (in 2.5 mM of phosphate buffer, pH 8.0 for gelatin based system and in 2.5 mM of borate buffer, pH 8.5 for chitosan based system).

Figure 2. Optimum temperature of gelatin (•) and chitosan (▪) based AChE electrode (in 2.5 mM of phosphate buffer, pH 8.0 for gelatin based system and in 2.5 mM of borate buffer, pH 8.5 for chitosan based system).

Figure 3. Effect of AChCl concentration on gelatin (a) and chitosan (b) based AChE electrode (in 2.5 mM of phosphate buffer, pH 8.0 for gelatin based system and in 2.5 mM of borate buffer, pH 8.5 for chitosan based system, at 25°C).

Figure 3. Effect of AChCl concentration on gelatin (a) and chitosan (b) based AChE electrode (in 2.5 mM of phosphate buffer, pH 8.0 for gelatin based system and in 2.5 mM of borate buffer, pH 8.5 for chitosan based system, at 25°C).

Figure 4. Operational (♦; 25°C) and thermal (•; 37°C) stability of chitosan based AChE electrode.

Figure 4. Operational (♦; 25°C) and thermal (•; 37°C) stability of chitosan based AChE electrode.

Table 1. Effects of organic solvents on chitosan based AChE electrode (incubation time; 30 and 60 min)

Table 2. Effects of organic solvents on gelatin based AChE electrode (incubation time; 30 and 60 min)

Figure 5. Inhibition curves of malathion (a), parathion-methyl (b), and methamidophos (c) for gelatin based AChE electrode.

Figure 5. Inhibition curves of malathion (a), parathion-methyl (b), and methamidophos (c) for gelatin based AChE electrode.

Figure 6. Inhibition curves of malathion (a), parathion-methyl (b), and methamidophos (c) for chitosan based AChE electrode.

Figure 6. Inhibition curves of malathion (a), parathion-methyl (b), and methamidophos (c) for chitosan based AChE electrode.

Table 3. Reproducibility of gelatin and chitosan based AChE biosensors

Table 4. Regeneration of chitosan based AChE electrode inhibited by various organophosphates; malathion, parathion-methyl, and methamidophos (PAM-2; 0.1 mM in 2.5 mM sodium borate buffer (pH 8.5), 60 min)

Table 5. Regeneration of gelatin based AChE electrode inhibited by various organophosphates (malathion, parathion-methyl, and methamidophos). PAM-2; 0.1 mM in 2.5 mM sodium borate buffer (pH 8.5), 60 min)

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