High-Porosity Activated Carbon as a Possible Matrix For Native DNA and Dextran-Sulfate Immobilization

Figures & data

Table 1. The influence of massive (7 mg/cm³) coating of mass-fractal carbonic matrices with DNA or DS on standard adsorption of free-diluted (creatinine, vitamin B₁₂) and protein-bound (bilirubun) substances (n = 6)

		Standard adsorption (mg/g)
Porosity by benzen (cm^3/g)	Coating	Creatinine	Vitamin B12	Bilirubin
1.85	No	54.8±2.3	162.0±5.5	3.58±0.21
	DNA	48.4±1.7a	128.0±5.2a	2.05±0.17a
	DS	45.1±2.2a	134.0±3.9a	2.18±0.32a
2.20	No	55.3±2.7	191.0±7.2	7.75±0.31
	DNA	52.7±4.1	175.0±6.3a	7.75±0.31
	DS	53.2±8.1	176.0±5.4a	6.40±0.48a
3.04	No	56.7±1.8	199.0±2.7	19.2±0.25
	DNA	54.7±1.9	196.0±3.9	18.6±0.97
	DS	53.1±2.7	198.0±4.2	19.0±0.53

^ap < 0.05 in comparation with appropriate uncoated carbonic matrix.

Table 2. Differences in concentration (Δ, % of initial level) of main groups of DNA-coupling substances in whole and diluted blood plasma after its perfusion through microcolumn containing mass-fractal adsorbents (γ = 0.1 g/cm³) covered with DNA or DS (0.7 mg of ligand per 1 g carbonic matrix) (n = 6)

		Types of plasma treatment
Coupling substances	Type of ligand	1	2	3	4	5
Anti-ds-DNA-ab	DNA	49.9±2.18	57.8±2.33a	59.5±0.75a	54.0±1.25	59.8±1.85a
	DS	51.3±2.13	50.4±3.44	59.3±0.92a	59.9±1.0a	76.2±0.69a
Anti-ss-DNA-ab	DNA	34.7±0.59	46.0±0.95a	47.25±0.50	50.5±2.2a	52.2±1.40a
	DS	45.4±0.89b	44.4±1.54	52.6±0.65a	57.8±0.83a	55.5±3.0a
Anti-DNP-ab	DNA	37.6±0.26	46.0±2.36a	47.7±0.75a	50.8±0.7a	53.6±0.42a
	DS	45.1±1.40a	44.2±1.41a	52.3±0.60a	57.8±1.37a	55.7±1.28a

^ap < 0.05 in comparation with results “1” of appropriate line.

^bp < 0.05 in comparation with results in the same bar of previous line.

1—Single-pass, 60 min pool.

2—Additional recirculation, 60 min pool.

3—Additional recirculation, 120 min pool.

4—2-times dilution, single-pass, 60 min pool.

5—5-times dilution, single-pass, 60 min pool.

Table 3. Residual concentrations of proinflammatory cytokines (initial is 1000 pg/mL of 3% BSA solution) after its contact with uncoated, DNA or DS-coated carbonic adsorbents (C.A.) or Adsorba 300C charcoal in single-pass microcolumn experiments

	Cytokines residual concentration (pg/mL)
Adsorbent samples	TNF-α	IL-1β	IL-6
C.A.	76.4±62.5	26.4±39.4	61.9±19.0
C.A. + DNA	21.3±15.7	0.5±0.95	14.7±10.5a
C.A. + DS	75.5±41.9	14.8±29.2	14.1±12.0a
Adsorba 300C	705.0±200.6	994.0±12.0	957.0±48.5

^ap < 0.05 in comparison with uncoated C.A.