Starch-hemoglobin Induces Contraction on Isolated Rat Aortic Rings

Figures & data

Table 1. Characteristics of the stroma-free and starch hemoglobin preparations used in the study

Parameters	Stroma-free hemoglobin	Starch hemoglobin
Hb (g/dL)	5.5±1.5	1.6±0.4
O2 (%)	96.9±2.8	86.8±13.0
COHb (%)	0.6±0.0	0.9±0.9
Methemoglobin (%)	1.4±2.1	4.5±1.4
O2 ct	6.5±3.3	1.5±1.1
SO2 (%)	98.4±0.9	100.54±1.6
O2 cap	6.7±3.4	1.5±1.2
RHb (%)	1.3±0.9	0.8±0.4

Data are mean ± S.D. of four samples.

Figure 1. Hb lane, SDS-polyacrylamide gel electrophoresis of stroma-free hemoglobin isolated by the selective DEAE-cellulose absorption method. MW lane, molecular weight markers.

Figure 2. Contraction induced by phenylephrine (10⁻⁶ M) in rings of aorta isolated from adult male rats. Bars represent mean (n = 10–12) and “T” lines represent S.E.M. *P < 0.01 as compared with preincubation value.

Figure 3. Concentration response curves to carbachol of rings of aorta isolated from adult male rats. Lines represent mean (n = 10–12) and “T” lines represent S.E.M.

Table 2. Relaxation response to carbachol of aortic rings after precontraction with phenylephrine 10⁻⁶ M

Previous incubation	Maximal response	EC50 (10^−7 M)
Stroma-free Hemoglobin	40.43±2.7a	10.23±2.81
Starch-hemoglobin	52.64±7.56a	42.31±24.71
Control (RKB)	79.68±1.84	3.98±0.43

Data are mean ± S.E.M.

^aP < 0.01 as compared with control incubation.