Figures & data
Figure 1 HPLC elution profile of BSA. The LS, RI and UV were the signals of laser light-scattering, refractive index and UV absorbance collected with Astra software, respectively.
Figure 2 HPLC elution profile of PEG-bHb as the molar ratio of PEG to bHb was 7:1. The LS, RI and UV were the signals of laser light-scattering, refractive index and UV absorbance collected with Astra software, respectively.
Figure 3 HPLC elution profile of PEG-bHb as the molar ratio of PEG to bHb was 10:1. The LS, RI and UV were the signals of laser light-scattering, refractive index and UV absorbance collected with Astra software, respectively.
Figure 4 HPLC elution profile of PEG-bHb as the molar ratio of PEG to bHb was 13:1. The LS, RI and UV were the signals of laser light-scattering, refractive index and UV absorbance collected with Astra software, respectivley.
Table 1 The average MCP and MP of three different PEG-bHb
Table 2 Relationship between molecular weight and elution time of F1
Table 3 Relationship between molecular weight and elution time of F2
Table 4 Relationship between molecular weight and elution time of F3
Figure 5 Molecular weight vs elution time of actived mPEG. The dashed lines represent the signal of laser, and the solid lines represent the signal of refractive index.
Figure 6 Molecular weight vs elution time of native bovine hemoglobin. The dashed lines represent the signal of laser, and the solid lines represent the signal of refractive index.
