BIODEGRADATION AND ADSORPTION OF PHENOL USING ACTIVATED CARBON IMMOBILIZED WITH PSEUDOMONAS PUTIDA

ABSTRACT

This paper examined the removal efficiency of phenol from aqueous solution using a suspended culture of Pseudomonas putida (ATCC 3180) or the activated carbon on which the microorganism was immobilized. The kinetics of phenol degradation by immobilized and pure cells was studied. Experiments were performed at various phenol concentrations (0.1–0.4 g/L), pH,7-9 temperature (30–36°C), and concentrations of glucose (0.5–0.7 g/L) and (NH₄)₂SO₄ (0.5–0.7 g/L). The presence of activated carbon markedly enhanced the degradation efficiency, showing its ability of protecting microbes from confronting shock loads of organic pollutants. Degradation rate increased with increasing substrate concentration and decreased after reaching a maximum, indicating substrate-inhibition kinetics. In addition, the degradation rate for immobilized cells was much higher than that of free cells. The inhibition effect for phenol degradation was described by the Andrews model. The kinetic parameters were also determined.

Keywords:

• Biodegradation
• Adsorption
• Phenol
• Pseudomonas putida
• Activated carbon

ACKNOWLEDGMENTS

Support for this work by the National Science Council, R.O.C., under the Grant No. NSC89-2211-002-008 is gratefully appreciated.