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Hemoglobin
international journal for hemoglobin research
Volume 25, 2001 - Issue 3
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COULD CAPILLARY ZONE ELECTROPHORESIS OF TRYPTIC PEPTIDES BE USED FOR THE CHARACTERIZATION OF HEMOGLOBIN VARIANTS?

Changying Lin Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
,
Béatrice Gulbis Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
,
Emile Delobbe Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
,
Patrick Robberecht Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
,
Frédéric Cotton Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
,
Bernard Fontaine Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
&
Françoise Vertongen Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
 show all
Pages 259-272 | Received 18 Aug 2000, Accepted 01 Jan 2001, Published online: 07 Jul 2009
 
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Abstract

The present article describes a simple and rapid new peptide mapping procedure that could be used to assist identification of rare hemoglobin variants in clinical laboratories. Four hemoglobin variants were taken as example, namely Hb D-Ouled Rabah, Hb Marseille, Hb G-Philadelphia, and Hb Ube-2, and isolated by electrophoresis at alkaline pH. The globin chains were aminoethylated and, after tryptic digestion, the peptides were separated by a capillary zone electrophoresis method. Highly reproducible migration times of the peptides were obtained with intra-assay and inter-assay coefficients of variation of less than 1 and 2%, respectively.

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