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Journal of Immunoassay and Immunochemistry Volume 25, 2004 - Issue 2
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Original Articles

Determination of (+)‐3,3′,5‐Triiodo‐L‐thyronine (L‐T3) from Serum Using a Sequential Injection Analysis/Immunosensor System

Raluca‐Ioana Stefan Department of Chemistry, University of Pretoria, Pretoria, 0002, South AfricaCorrespondence[email protected]
,
Jacobus Frederick van Staden Department of Chemistry, University of Pretoria, Pretoria, 0002, South Africa
&
Hassan Y. Aboul‐Enein Pharmaceutical Analysis and Drug Development Laboratory, Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia
Pages 183-189 | Received 10 Nov 2003, Accepted 29 Nov 2003, Published online: 18 Aug 2006
 
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Abstract

A sequential injection analysis/immunosensor system is proposed for the analysis of T3 in serum with a rate of 75 samples/hr. The immunosensor design is based on the physical immobilization of anti‐T3 in carbon paste. The working concentration range of the immunosensor in a sequential injection analysis system is between 3.4 and 340 ng/mL with a limit of detection of 2.19 ng/mL. The system is very reliable and very easy to design and operate.

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