Figures & data
Figure 1A. Solution hybridization assay for in control and mercuric chloride (HgCl2) EGF injected rats at days 1 and 3. The probe is shown diluted 1:10 and 1:100. Simultaneous hybridization was carried out with 18S shown in the lower panel.
![Figure 1A. Solution hybridization assay for in control and mercuric chloride (HgCl2) EGF injected rats at days 1 and 3. The probe is shown diluted 1:10 and 1:100. Simultaneous hybridization was carried out with 18S shown in the lower panel.](/cms/asset/adbd3cfc-5fd3-488c-aaa2-81ff35555276/irnf_a_11378863_uf0001_b.gif)
Figure 2. Gel of the probe and protected fragments after solution hybridization for heparin binding EGF at day 3 in controls and mercuric chloride treated rats. 18S is presented in the lower panel.
![Figure 2. Gel of the probe and protected fragments after solution hybridization for heparin binding EGF at day 3 in controls and mercuric chloride treated rats. 18S is presented in the lower panel.](/cms/asset/e10b4a2d-a384-437a-9464-2fd6243f67b7/irnf_a_11378863_uf0003_b.gif)
Figure 3A. Gel of the probes and protected fragments for TGF-α and the EGF receptor 3 days following vehicle injection or HgCl2 4 mg/kg. A standard curve was prepared from normal rat kidneys; 18S was hybridized as well in these assays.
![Figure 3A. Gel of the probes and protected fragments for TGF-α and the EGF receptor 3 days following vehicle injection or HgCl2 4 mg/kg. A standard curve was prepared from normal rat kidneys; 18S was hybridized as well in these assays.](/cms/asset/a5304fe6-8e53-4363-9ef9-9b098728bd56/irnf_a_11378863_uf0004_b.gif)
Figure 3B. Data normalized for background and sample loading for TGF-α mRNA. Four animals were studied in each group. * p < 0.05.
![Figure 3B. Data normalized for background and sample loading for TGF-α mRNA. Four animals were studied in each group. * p < 0.05.](/cms/asset/3c89a595-df0b-406a-9a9d-a7dfbcc1dd7b/irnf_a_11378863_uf0005_b.gif)
Figure 3C. Corrected data for EGF receptor mRNA in the various groups of control and mercuric chloride treated rats. Four animals were studied in each group. * p < 0.01.
![Figure 3C. Corrected data for EGF receptor mRNA in the various groups of control and mercuric chloride treated rats. Four animals were studied in each group. * p < 0.01.](/cms/asset/3b91bb3b-3884-4486-b5cd-ba75e6ca7481/irnf_a_11378863_uf0006_b.gif)
Figure 4. Immunohistochemistry for TGF-α. (A) Injury section stained with control serum alone. (B) control kidney section stained for TGF-α. (C) Cortical section from a rat treated with HgCl2 4 mg/kg at day 3. Staining was confined to distal tubules in control kidneys whereas it was more pronounced following the induction of injury. Similar observations were made in multiple other sections from 5 animals.
![Figure 4. Immunohistochemistry for TGF-α. (A) Injury section stained with control serum alone. (B) control kidney section stained for TGF-α. (C) Cortical section from a rat treated with HgCl2 4 mg/kg at day 3. Staining was confined to distal tubules in control kidneys whereas it was more pronounced following the induction of injury. Similar observations were made in multiple other sections from 5 animals.](/cms/asset/0d49e77b-37f3-4e2d-9bf9-af1c04410207/irnf_a_11378863_uf0007_b.gif)