TUBULAR CELL LESION, ALBUMINURIA, AND RENAL ALBUMIN HANDLING IN RATS TREATED WITH ADRIAMYCIN

Figures & data

Figure 4. Urinary albumin excretion before and after the treatment with 5 mg/kg adriamycin (AD) or with 5 mg/kg adriamycin plus sodium bicarbonate (AD-B). Data are expressed as mean ± SD.

Table 1. Albuminuria and Immunohistochemical Data for the Renal Cortex from Control Rats and from Rats Killed 7 (AD 7 D) and 18 Days (AD 18 D) After Adriamycin Injection (3.5 mg/kg)

Rats	Albuminuria (mg/24H)	Albumin (Score)	Vimentin (Score)	PCNA (cells +/0.36 mm^2)
Control	0.31 ± 0.22	0.20 ± 0.14	0.04 ± 0.03	4.78 ± 1.22
AD 7 D	0.33 ± 0.03	0.18 ± 12	0.01 ± 0.01	4.19 ± 1.06
AD 18 D	71.4 ± 37.4^**	1.74 ± 0.29^**	0.36 ± 0.39^*	23.5 ± 17.1^*

Values are means ± SD,

^*P < 0.05;

^**P < 0.001 vs control.

Figure 1. Immunolocalization of albumin in the renal cortex of a rat killed on day 18 after treatment with 3.5 mg/kg adriamycin (B) and of a control rat (A). Note that the immunostaining in the cytoplasm of the tubular cells is more intense in B. ×420.

Figure 2. Immunolocalization of vimentin in the renal cortex of a rat killed on day 18 after treatment with 3.5 mg/kg adriamycin (B) and of a control rat (A). Note that the immunostaining in tubular cells is more intense in B. ×420.

Figure 5. Immunostaining score for vimentin for control rats (C, N = 7), for rats killed on day 18 after treatment with 5 mg/kg adriamycin (AD, N = 7) and for rats killed on day 18 after treatment with 5 mg/kg adriamycin plus sodium bicarbonate (AD-B, N = 6). The median is shown as a horizontal line.

Figure 3. Immunolocalization of PCNA in the renal cortex of a rat killed on day 18 after treatment with 3.5 mg/kg adriamycin (B) and of a control rat (B). Observe that the amount of PCNA positive cells is higher in B. ×420.