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Abstract
Plasma homocysteine level has been linked with the risk of occlusive vascular disease. Due to the presence of multiple forms of homocysteine, with the majority found as protein-bound homocysteine, and the interchanges among non-protein-bound homocysteines, the concern of analytical reliability for total plasma homocysteine has been raised. In this research, plasma samples are reduced by dithiothreitol to break disulfide linkages.
The reduced samples are separated and analyzed for homocysteine and its related compounds by ion-exchange chromatography with post-column ninhydrin derivatization and spectrometric detection. The total homocysteine of plasma is determined as the sum of homocysteine, homocysteine thiolactone, homocystine, and cysteine-homocysteine disulfide.
ACKNOWLEDGMENTS
The support of this work by the College of New Jersey through the FIRSL grant is gratefully acknowledged. Also, the authors would like to acknowledge Chambers Clinical Laboratories (Trenton, New Jersey) for allowing the use of their facilities for this research.