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Abstract
Cyanobacteria (blue-green algae) have been shown to produce unique biologically active peptides such as microcystins, mainly from different species of Microcystis aeruginosa. Presence of these toxic peptides (hepatotoxin) in fresh water supplies are of increasing concern and has emphasized the need for a suitable analytical method. Amounts of microcystin-LR were estimated in the laboratory cultures of PCC strains viz. PCC-7806, 7820, and PCC-7941, using high performance liquid chromatography. The identity of microcystin-LR was confirmed by LC-ESI-MS. The lower detection limit of quantification is 1.0 μg/L.
ACKNOWLEDGMENTS
We thank Dr. R.V. Swamy, Chief Controller, Defense Research and Development Organization, New Delhi, for his encouragement and helpful advice. We are also thankful to Prof. Wayne. W. Carmichael of Wright State University Dayton, Ohio, USA, for providing the standard reference and Dr. P.V.L. Rao of DRDE Gwalior, for providing the cultured samples. M.N. is indebted to DRDO New Delhi for financial support.