MOLECULAR MODELING OF ENANTIOMERIC RESOLUTION OF METHYLPHENIDATE ON CELLULOSE TRISBENZOATE CHIRAL STATIONARY PHASE

ABSTRACT

The enantiomeric resolution of (±)-threo methylphenidate (MPH) (Ritalin®) has been achieved on Chiralcel OB column using the mobile phase hexane–ethanol–methanol–trifluoroacetic acid (480 : 9.75 : 9.75 : 0.5, v/v/v/v), containing benzoic acid and phenol as mobile phase additives. Ultraviolet detection was carried out at 230 nm. Molecular modeling was carried out to explain the chiral resolution mechanism of MPH enantiomers.

ACKNOWLEDGMENT

The authors (I.A. and H.Y.A-E.) would like to thank the King Faisal Specialist Hospital and Research Center, Riyadh administration for their support for the Pharmaceutical Analysis Laboratory Research Program.