SYNTHESIS AND CHARACTERIZATION OF A C₈ STATIONARY PHASE, BONDED WITH 2-ACRYLAMIDO-2-METHYL-1-PROPANESULFONIC ACID, FOR HPLC

ABSTRACT

The silanization/hydrosilation method is used to bond a mixed stationary phase consisting of both octyl (C₈) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) moieties. The intermediates, as well as the final product, are characterized by elemental analysis, diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy, and carbon-13 CP-MAS NMR spectroscopy. The results demonstrate that both the AMPS and C₈ groups are successfully bonded on the same silica hydride support material. The final product (C₈+AMPS) is characterized chromatographically and compared to the behavior of the same solutes on both a C₈ phase and an AMPS phase.

ACKNOWLEDGMENTS

The authors would like to thank the National Institutes of Health (NIH MBRS Grant 0819210-21) and the National Science Foundation (CHE 0094983) for support of the research in this study. One author (JJP) greatly acknowledges the support of the Camille and Henry Dreyfus Foundation through a Scholar Award.