Estimation of Separation Factor for Analogues in CCC Using UNIFAC

Abstract

Separation factor is an important index for judging the eluotropic sequence of solutes in countercurrent chromatography (CCC). This paper has proven that the contribution of the common basic agglomerate can be eliminated when calculating the separation factor for analogues and introduced a computational equation based on UNIFAC. According to the equation, the estimation of separation factors needs only a few different groups of analogues. Eleven kinds of analogues, such as phytosterol, chlorinated s‐triazines, flavonoids, theaflavins, anthocyanins, sporaviridins, etc., have been tested with the method. Though the solvent systems used are different from each other, the estimated eluotropic sequences are all consisted well with the literatures except for two chlorinated s‐triazines.

Keywords:

• Eluotropic sequence
• Separation factor
• Analogue
• Countercurrent chromatography
• UNIFAC