Abstract
The preparation of 12 National Certified Reference Materials of active compounds from natural products with countercurrent chromatography (CCC) are reported in this paper. They are kaempferol (1), isorhamnetin (2), quercetin (3), icariin (4), epigallocatechin‐3‐o‐gallate (EGCG) (5), epicatechin‐3‐o‐gallate (ECG) (6), gallocatechin‐3‐o‐gallate (GCG) (7), resveratrol (8), piceid (9), 2,3,5,4′‐tetra hydroxystibene‐2‐o‐D‐glucoside (10), puerarin (11), 3′‐methoxy‐puerarin (12). All of their certified purities were higher than 98%.
Acknowledgments
Support from the State Bureau of Quality and Technical Inspection and financial support from Zhejiang Recover Biotech Co., LTD. are gratefully acknowledged. The authors also would like to thank Mr. Qinghui Liu for the preparation of CRMs. We are also grateful for the advice from Prof. Guangwei Zhang and Prof. Bonian Chen of China Association for Standardization CSBTS.