Countercurrent Chromatographic Purification of Polysaccharides from Achyranthes bidentata with an Aqueous Two‐Phase System Using a Cross‐Axis Coil Planet Centrifuge

Abstract

Polysaccharides were purified from a high molecular weight complex of Achyranthes bidentata Bl. (Fam. Amaranthaceae) root by high speed countercurrent chromatography (HSCCC) using a cross‐axis coil planet centrifuge (X‐axis CPC). The purification was performed with an aqueous polymer two‐phase system composed of 12.5% (w/w) polyethylene glycol (PEG) 1000, 8% (w/w) potassium dihydrogen phosphate, and 8% (w/w) dipotassium hydrogen phosphate buffer at pH 6.8. The preparative separation of the crude sample (2 g) was successfully performed, yielding 1.1 g of polysaccharides at high purity.

Keywords:

• Countercurrent chromatography
• Cross‐axis coil planet centrifuge
• Polysaccharides
• Partition coefficient

Acknowledgment

This work was financially supported by China Scholarship Council fellowship (No. 99361117).