Determination of Famotidine in Acid Reduction Tablets by HPTLC and Videodensitometry of Fluorescence Quenched Zones

Abstract

A quantitative method using silica gel high performance thin layer chromatography (HPTLC) plates with fluorescent indicator, automated sample application, and ultraviolet (UV) absorption videodensitometry was developed for the determination of famotidine tablets, which are widely used to inhibit gastric acid secretion. Three pharmaceutical tablet products containing famotidine as the active ingredient were analyzed to test the applicability of the new method. Precision was evaluated by replicate analyses of the samples and accuracy by analysis of a sample, fortification with standard, and reanalysis (standard addition). The percent famotidine in the tablets ranged from 92.5% to 140% compared to label values, precision from 1.25% to 2.55% relative standard deviation, and the error in the standard addition analysis was 1.76% compared to the fortification level. These validation results are within the guidelines of the International Conference on Harmonization for pharmaceutical analysis.

Keywords:

• High performance thin layer chromatography
• Determination of famotidine
• Videodensitometry
• Pharmaceutical analysis

Acknowledgment

Alison N. Campbell was supported by an EXCEL Scholar Grant from the Academic Research Committee of Lafayette College.