Abstract
α‐Chymotrypsin (ACHT) was immobilized on an epoxide‐derivatized silica gel by an in situ immobilization process. Several racemates has been resolved according an enzymatic recognition mechanism on this ACHT chiral stationary phase. The loadability of the ACHT‐CSP, used as bioreactor, has been evaluated in both buffered and non‐buffered mobile phases under the enzymatic process.
Notes
aThe C3 stationary phase is slightly hydrophobic, so able to separate the ester and the acid forms with a mobile phase totally buffered.