Abstract
The intra- and intermolecular hydrogen bonding (ΔGº298K ≈ 2, kcal mol−1) of 2′-OH in nucleos(t)ides has been reported by the temperature- and concentration-dependent NMR study in conjunction with dihedral dependence of the NMR derived both endo (3 J H,H)- and exocyclic (3 JH,OH) coupling constants, nOe contacts and lineshape analyses of hydroxyl protons for EtpA (1), 3′-dA (2), rA (3), 2′-dA (4) [Fig. ] in DMSO-d 6 at 500 MHz.
ACKNOWLEDGEMENTS
We thank the Swedish Board for Technical Development (TFR), the Swedish Natural Science Research Council (NFR), and the Swedish Board for Technical Development (NUTEK) for generous financial support. Thanks are due to the Wallenbergstiftelsen, ForskningsraÊ dsnämnden and the University of Uppsala for funds to purchase of 500-MHz and 600-MHz Bruker DRX NMR spectrometers.