Oxidative DNA Cleavage by Free and Oligonucleotide‐Conjugated O‐Bromobenzoic Acid in the Presence of Copper(II) Ions

Abstract

o‐Bromobenzoic acid was found to promote copper‐dependent reactive oxygen species formation from molecular oxygen, resulting in DNA base modification and backbone cleavage. The oligonucleotide conjugate bearing 5‐(4′‐aminopropyl‐sulfomoyl)‐2‐bromobenzoic acid as a reactive group was synthesized and DNA cleavage activity of this oligonucleotide conjugate was tested on a model deoxyoligonucleotide.

Keywords:

• Metallocomplexes
• DNA cleavage
• Oligonucleotide conjugates

Acknowledgment

The research was supported by Russian Government Program “Gene‐directed biologically active compounds.”