COMPARISON OF GAS CHROMATOGRAPHY AND IMMUNOASSAY METHODS IN MEASURING THE DISTRIBUTION OF DIELDRIN IN RAINBOW TROUT TISSUES

Abstract

Studies have been conducted to determine the distribution of dieldrin in various tissues of rainbow trout when exposed to several dieldrin concentrations. Medium sized fish with an average weight and length of 195.4 ± 30.5 g and 25.7 ± 1.4 cm, respectively, were placed in groups of 6 in 300 L tanks containing purified and aerated water and maintained at 10°C. Following an acclimatization period of 10 days, each group of fish was exposed to one of four dieldrin concentrations ranging from 50 to 80 ppb. After 24 hours, the fish were taken out of the tanks and sacrificed. The brain, gills, liver, muscles and skin were collected from each fish. Dieldrin was extracted from each tissue using SPE techniques and analyzed by both gas chromatography (GC) and enzyme linked immunosorbent assay (ELISA). Results of analyses by the two techniques were highly correlated. The results also showed that liver and skin tissues had the highest level of dieldrin residues. In comparing the means of the six fish samples, it was found that liver or skin contained about 1.5-fold the level in brain, about 4.0 fold the level in muscles and about 6.5 fold the level in gills. Immunoassay proved to be as reliable an analytical tool as gas chromatography in this case.

Keywords:

• Fish
• Dieldrin
• Gas chromatography
• Immunoassay
• Insecticide
• Rainbow trout

ACKNOWLEDGMENTS

This study was supported by a research grant from the University Research Board, American University of Beirut. The technical assistance of Mr.= Nabil Nemer is gratefully acknowledged.