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Spectroscopy Letters
An International Journal for Rapid Communication
Volume 36, 2003 - Issue 3
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Original Articles

Mechanism of the Interaction Between Bromophenol Blue and Bovine Serum Albumin

W. G. Cao Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, 210093, Nanjing, P.R. China; Department of Biochemistry, Suzhou Teachers' College, 234000, Suzhou, Anhui, P.R. China
,
Q. C. Jiao Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, 210093, Nanjing, P.R. ChinaCorrespondence[email protected]
,
Y. Fu Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, 210093, Nanjing, P.R. China
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L. Chen Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, 210093, Nanjing, P.R. China
&
Q. Liu Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, 210093, Nanjing, P.R. China
Pages 197-209 | Received 01 Nov 2002, Accepted 28 Mar 2003, Published online: 15 Aug 2006
 
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Abstract

The mechanism of the interaction between Bromophenol Blue (BPB) and Bovine Serum Albumin (BSA) was studied spectrophotometrically. Absorption spectral changes of BPB–BSA and BPB–Chitosan mixtures in different BSA and Chitosan concentrations are discussed. The influence of different experimental conditions on the absorption spectra of BPB–BSA mixtures was investigated. Through these experiments, the conclusion was drawn that the bathochromic shift of the absorption spectra of the BPB–BSA reaction system results mainly from hydrophobic interaction between BPB and BSA, while electrostatic force is necessary for the formation of the BPB–BSA complex.

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