Abstract
The differences in the backbone conformation between O‐thymidine‐3′‐(1) and 5′‐yl O‐alkyl N‐phosphoryl serine methyl esters (2) have been investigated by solution 13C NMR spectroscopy. The stereo‐sensitive vicinal 31P–13C coupling constants were measured and used in the conformational analysis for the P–O5′–C5′, P–O3′–C3′, and P–N–Cα bonds. Three‐dimensional structural characteristics of dephosphorylation reactions of Compounds are also discussed.
Acknowledgment
The authors would like to extend thanks for financial support from the Chinese National Natural Science Foundation (No. 20132020), the Ministry of Science and Technology, the Chinese Ministry of Education and Tsinghua University.