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Papers

Evaluation of synovium-derived mesenchymal stem cells and 3D printed nanocomposite scaffolds for tissue engineering

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Article: 045001 | Received 23 Mar 2015, Accepted 15 Jun 2015, Published online: 16 Jul 2015

Figures & data

Table 1. Composition of 3D nanocomposite scaffolds.

Figure 1. Quantitative flow cytometry analysis of typical cell surface markers (red color) from mesenchymal stem cell (CD44, CD90, and CD105) (a)–(c) and hematopoietic stem cell (CD45) (d).

Figure 1. Quantitative flow cytometry analysis of typical cell surface markers (red color) from mesenchymal stem cell (CD44, CD90, and CD105) (a)–(c) and hematopoietic stem cell (CD45) (d).

Figure 2. Multilineage differentiation of SMSCs. (a) After incubation in chondrogenic medium SMSCs were positively stained for collagen II protein (immunostaining). (b) After incubation in adipogenic medium SMSCs were positively stained for adipose drops (Oil Red O). (c) After incubation in osteogenic medium SMSCs were positively stained for calcium matrix (Alizarin Red).

Figure 2. Multilineage differentiation of SMSCs. (a) After incubation in chondrogenic medium SMSCs were positively stained for collagen II protein (immunostaining). (b) After incubation in adipogenic medium SMSCs were positively stained for adipose drops (Oil Red O). (c) After incubation in osteogenic medium SMSCs were positively stained for calcium matrix (Alizarin Red).

Figure 3. SEM images of gelatin nanoparticles.

Figure 3. SEM images of gelatin nanoparticles.

Figure 4. 3D printed nanocomposite scaffolds. (a) NP/SF, (b) NP/SF–Gel, (c) NP/Gel-1, (d) NP/Gel-2.

Figure 4. 3D printed nanocomposite scaffolds. (a) NP/SF, (b) NP/SF–Gel, (c) NP/Gel-1, (d) NP/Gel-2.

Figure 5. SEM images of 3D printed nanocomposite scaffolds. (a) NP/SF, (b) NP/SF–Gel, (c) NP/Gel-1, (d) NP/Gel-2.

Figure 5. SEM images of 3D printed nanocomposite scaffolds. (a) NP/SF, (b) NP/SF–Gel, (c) NP/Gel-1, (d) NP/Gel-2.

Figure 6. The proliferation of SMSCs cocultured with 3D printed nanocomposite scaffolds in the CCK-8 assay: the absorbance of these medium with CCK-8 was read at 450 nm.

Figure 6. The proliferation of SMSCs cocultured with 3D printed nanocomposite scaffolds in the CCK-8 assay: the absorbance of these medium with CCK-8 was read at 450 nm.

Figure 7. Microscopy images of SMSCs cocultured with 3D printed nanocomposite scaffolds shown by red arrows (a) NP/SF, (b) NP/SF–Gel, (c) NP/Gel-1, (d) NP/Gel-2.

Figure 7. Microscopy images of SMSCs cocultured with 3D printed nanocomposite scaffolds shown by red arrows (a) NP/SF, (b) NP/SF–Gel, (c) NP/Gel-1, (d) NP/Gel-2.