Figures & data
Figure 1 (a,b) 52Mn translocation from the roots of (a) Mn-deficient and (b) Mn-sufficient (control) barley plants. Images were obtained by autoradiography. Rectangles show the area measured by the positron-emitting tracer imaging system (PETIS). DC, discrimination center; YL, youngest leaf. Scale bars are 4 cm. These images cannot be compared to each other. (c) Time-course of radioactivity in Mn-deficient (left) and control (right) barley as measured by PETIS. The images were taken at 15-min and 2-h intervals (0–120 min and 2–8 h, respectively). Arrowheads indicate the DC. Scale bars are 1 cm. (d) Time-course of radioactivity in the DC of Mn-deficient (purple dots) and control (orange dots) barley. The maximum measured radioactivity was defined as 100, and all measurements were converted relative to the maximum. (e) Time-course of radioactivity in the DC and at a position 3.41 cm above the DC in the main shoot of Mn-deficient barley (indicated as “shoot” in [c]). Arrows indicate the arrival time of 52Mn. The arrival time was defined as the time at which radioactivity began to be detected.
![Figure 1 (a,b) 52Mn translocation from the roots of (a) Mn-deficient and (b) Mn-sufficient (control) barley plants. Images were obtained by autoradiography. Rectangles show the area measured by the positron-emitting tracer imaging system (PETIS). DC, discrimination center; YL, youngest leaf. Scale bars are 4 cm. These images cannot be compared to each other. (c) Time-course of radioactivity in Mn-deficient (left) and control (right) barley as measured by PETIS. The images were taken at 15-min and 2-h intervals (0–120 min and 2–8 h, respectively). Arrowheads indicate the DC. Scale bars are 1 cm. (d) Time-course of radioactivity in the DC of Mn-deficient (purple dots) and control (orange dots) barley. The maximum measured radioactivity was defined as 100, and all measurements were converted relative to the maximum. (e) Time-course of radioactivity in the DC and at a position 3.41 cm above the DC in the main shoot of Mn-deficient barley (indicated as “shoot” in [c]). Arrows indicate the arrival time of 52Mn. The arrival time was defined as the time at which radioactivity began to be detected.](/cms/asset/9500275f-15f5-4ab8-bfb0-6a4439038958/tssp_a_10382448_o_f0001g.gif)
Figure 2 (a,b) 52Mn translocation from the roots of (a) Mn-excess and (b) control barley. Images were obtained by autoradiography. Rectangles show the area measured by the positron-emitting tracer imaging system (PETIS). Scale bars are 4 cm. These images cannot be compared to each other. (c) Time-course of radioactivity in Mn-excess (left) and control (right) barley as measured by PETIS. The images were taken at 1-h intervals. DC, discrimination center. Scale bars are 1 cm. (d) Time-course of radioactivity in the DC of Mn-excess (purple dots) and control (orange dots) barley. The maximum measured radioactivity was defined as 100, and all measurements were converted relative to the maximum.
Figure 3 (a,b) 52Mn translocation from the roots of control barley in (a) darkness and (b) light. Images were obtained by autoradiography. Leaf numbering: 4, the second-youngest leaf on the main shoot; 3, the third leaf; 2, the second leaf; 1, the first leaf. The youngest leaf, the fifth leaf, is present inside the leaf sheath. DC, discrimination center. Scale bars are 4 cm. (c) Time-course of radioactivity in the DC (as marked in [a,b]) of barley in darkness (•) and light (○). The maximum measured radioactivity was defined as 100, and all measurements were converted relative to the maximum. (d) 52Mn content in the DC and leaves of barley in darkness (▪) and light (□).
![Figure 3 (a,b) 52Mn translocation from the roots of control barley in (a) darkness and (b) light. Images were obtained by autoradiography. Leaf numbering: 4, the second-youngest leaf on the main shoot; 3, the third leaf; 2, the second leaf; 1, the first leaf. The youngest leaf, the fifth leaf, is present inside the leaf sheath. DC, discrimination center. Scale bars are 4 cm. (c) Time-course of radioactivity in the DC (as marked in [a,b]) of barley in darkness (•) and light (○). The maximum measured radioactivity was defined as 100, and all measurements were converted relative to the maximum. (d) 52Mn content in the DC and leaves of barley in darkness (▪) and light (□).](/cms/asset/7ec94e14-5200-4d65-a76a-2ccd242bff0c/tssp_a_10382448_o_f0003g.gif)
Figure 4 (a) 52Mn translocation from a leaf tip (arrowhead) to other tissues in a control barley plant. Images were obtained by autoradiography. Leaf numbering: 4, the youngest leaf on the main shoot; 3, the third leaf; 2, the second leaf; 1-2, 1-1, younger and older leaf on the first tiller, respectively. Scale bar is 4 cm. (b) Time-course of radioactivity in the discrimination center (DC). The maximum measured radioactivity was defined as 100, and all measurements were converted relative to the maximum.
