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ORIGINAL ARTICLE

Cadmium uptake in barley affected by iron concentration of the medium: Role of phytosiderophores

, &
Pages 259-266 | Received 04 Sep 2006, Accepted 07 Jan 2007, Published online: 17 Dec 2010

Figures & data

Figure 1  Effect of Fe deficiency on (a) dry weights, (b) Cd concentrations and (c) Fe concentrations in the shoots and roots of barley plants grown in media with 5 µmol L−1 CdSO4. Each value is the mean ± standard error (n = 4) and different letters at the top of each bar indicate significant differences (P < 0.05) according to the Ryan–Einot–Gabriel–Welsch multiple range test. d.w., dry weight.

Figure 1  Effect of Fe deficiency on (a) dry weights, (b) Cd concentrations and (c) Fe concentrations in the shoots and roots of barley plants grown in media with 5 µmol L−1 CdSO4. Each value is the mean ± standard error (n = 4) and different letters at the top of each bar indicate significant differences (P < 0.05) according to the Ryan–Einot–Gabriel–Welsch multiple range test. d.w., dry weight.

Figure 2  Effect of excess Fe on (a) dry weights, (b) Cd concentrations and (c) Fe concentrations in the shoots and roots of barley plants grown in media with 5 µmol L−1 CdSO4. Each value is the mean ± standard error (n = 4) and different letters at the top of each bar indicate significant differences (P < 0.05) according to the Ryan–Einot–Gabriel–Welsch multiple range test. d.w., dry weight.

Figure 2  Effect of excess Fe on (a) dry weights, (b) Cd concentrations and (c) Fe concentrations in the shoots and roots of barley plants grown in media with 5 µmol L−1 CdSO4. Each value is the mean ± standard error (n = 4) and different letters at the top of each bar indicate significant differences (P < 0.05) according to the Ryan–Einot–Gabriel–Welsch multiple range test. d.w., dry weight.

Figure 3  Solubilized amount shown as the concentration of Cd and Fe in the filtrated solutions by mugineic acid (MA) from mixtures of Cd and Fe gel. Cadmium gel and Fe gel were formed by precipitation in the presence of Na2S. Each value is the mean ± standard error (n = 4) and different letters at the top of each bar indicate significant differences (P < 0.05) according to the Ryan–Einot–Gabriel–Welsch multiple range test. ND, not detected.

Figure 3  Solubilized amount shown as the concentration of Cd and Fe in the filtrated solutions by mugineic acid (MA) from mixtures of Cd and Fe gel. Cadmium gel and Fe gel were formed by precipitation in the presence of Na2S. Each value is the mean ± standard error (n = 4) and different letters at the top of each bar indicate significant differences (P < 0.05) according to the Ryan–Einot–Gabriel–Welsch multiple range test. ND, not detected.

Figure 4  Effect of feeding mugineic acid (MA) on Cd concentrations of the shoots and roots. (a) Plants grown in the medium without Fe were transferred to Fe-deficient medium containing 5 µmol L−1 CdSO4 without (0 µmol L−1) or with (10 µmol L−1) MA and kept for 4 h, and (b) plants grown in the medium with Fe were transferred to Fe-sufficient medium containing 5 µmol L−1 CdSO4 without (0 µmol L−1) or with (10 µmol L−1) MA and kept for 4 h. Each value is the mean ± standard error (n = 4) and different letters at the top of each bar indicate significant differences (P < 0.05) according to the Ryan–Einot–Gabriel–Welsch multiple range test.

Figure 4  Effect of feeding mugineic acid (MA) on Cd concentrations of the shoots and roots. (a) Plants grown in the medium without Fe were transferred to Fe-deficient medium containing 5 µmol L−1 CdSO4 without (0 µmol L−1) or with (10 µmol L−1) MA and kept for 4 h, and (b) plants grown in the medium with Fe were transferred to Fe-sufficient medium containing 5 µmol L−1 CdSO4 without (0 µmol L−1) or with (10 µmol L−1) MA and kept for 4 h. Each value is the mean ± standard error (n = 4) and different letters at the top of each bar indicate significant differences (P < 0.05) according to the Ryan–Einot–Gabriel–Welsch multiple range test.

Figure 5  Amount of phytosiderophores (PS) released from the roots of barley plants grown in Fe-deficient media with 0, 0.05, 0.5 or 5 µmol L−1 CdSO4. Each value is the mean ± standard error (n = 3) and different letters at the top of each bar indicate significant differences (P < 0.05) according to the Ryan–Einot–Gabriel–Welsch multiple range test.

Figure 5  Amount of phytosiderophores (PS) released from the roots of barley plants grown in Fe-deficient media with 0, 0.05, 0.5 or 5 µmol L−1 CdSO4. Each value is the mean ± standard error (n = 3) and different letters at the top of each bar indicate significant differences (P < 0.05) according to the Ryan–Einot–Gabriel–Welsch multiple range test.

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