Overexpression of the LASAP2 gene for secretory acid phosphatase in white lupin improves the phosphorus uptake and growth of tobacco plants

Figures & data

Figure 1  Plant growth of tobacco LASAP2 line (L2) and wild-type (WT) plants in sterilized media. The gray and black bars indicate the shoots and roots, respectively. Values in parentheses indicate the root-to-shoot ratios. Data are presented as the mean ± standard error (n = 5). Significant differences between WT and L2 line in each treatment are indicated by *(P < 0.01).

Figure 2  Phosphorus contents of the tobacco LASAP2 line (L2) and wild-type (WT) plants grown in sterilized media. The gray and black bars indicate the shoots and roots, respectively. Values in parentheses indicate the root-to-shoot ratios. Data are presented as the mean ± standard error (n = 5). Significant differences between WT and L2 line in each treatment are indicated by *(P < 0.01).

Figure 3  Plant growth of the tobacco LASAP2 line (L2) and wild-type (WT) plants in sterilized media. The gray and black bars indicate the shoots and roots, respectively. Values in parentheses indicate root-to-shoot ratios. Data are presented as the mean ± standard error (n = 5). Significant differences between WT and L2 line in each treatment are indicated by *(P < 0.01).

Table 1 Total P and available P concentrations in the prepared soils. Different letters indicate significant differences (P<0.01)

Treatment	P concentration (mg P g soil^−1)
	+P	Phytate	–P
Total-P	1.383 ± 0.033a	1.298 ± 0.051ab	1.208 ± 0.011b
Truog-P	0.109 ± 0.031a	0.031 ± 0.000b	0.031 ± 0.000b

Figure 4  Shoot P contents of the tobacco LASAP2 line (L2) and wild-type (WT) plants grown in sterilized media. Values in parentheses indicate the root-to-shoot ratios. Data are presented as the mean ± standard error (n = 5).

Figure 5  Relationship between acid phosphatase (APase) activity in the rhizosphere soil and P accumulation.