Abstract
Polypyrimidine tract binding protein (PTB) is a widely expressed RNA binding protein. In the nucleus PTB regulates the splicing of alternative exons, while in the cytoplasm it can affect mRNA stability, translation, and localization. Here we demonstrate that PTB transiently localizes to the cytoplasm and to protrusions in the cellular edge of mouse embryo fibroblasts during adhesion to fibronectin and the early stages of cell spreading. This cytoplasmic PTB is associated with transcripts encoding the focal adhesion scaffolding proteins vinculin and alpha-actinin 4. We demonstrate that vinculin mRNA colocalizes with PTB to cytoplasmic protrusions and that PTB depletion reduces vinculin mRNA at the cellular edge and limits the size of focal adhesions. The loss of PTB also alters cell morphology and limits the ability of cells to spread after adhesion. These data indicate that during the initial stages of cell adhesion, PTB shuttles from the nucleus to the cytoplasm and influences focal adhesion formation through coordinated control of scaffolding protein mRNAs.
SUPPLEMENTAL MATERIAL
Supplemental material for this article may be found at http://mcb.asm.org/ .
ACKNOWLEDGMENTS
We thank Geetanjali Chawla for the shPTB(B) construct, Kris DeMali for the vinculin null MEF cell line, Steven Smale for the RelA−/− MEF cell line, Arnold Berk for the SV40-transformed MEF cell line, Gideon Dreyfuss for the anti-hnRNP K antibody, and Brigitte Jockusch for the anti-Raver antibody.
I.B. was supported by a fellowship from the Alberta Heritage Foundation for Medical Research. This work was supported in part by NIH grant RO1GM49662 to D.L.B. D.L.B. is an Investigator of the Howard Hughes Medical Institute.