Abstract
Human transcription factor IIIC (hTFIIIC) is a multisubunit complex that directly recognizes promoter elements and recruits TFIIIB and RNA polymerase III. Here we describe the cDNA cloning and characterization of the 90-kDa subunit (hTFIIIC90) that is present within a DNA-binding subcomplex (TFIIIC2) of TFIIIC. hTFIIIC90 has no specific homology to any of the known yeast TFIIIC subunits. Immunodepletion and immunoprecipitation studies indicate that hTFIIIC90 is a bona fide subunit of TFIIIC2 and absolutely required for RNA polymerase III transcription. hTFIIIC90 shows interactions with the hTFIIIC220, hTFIIIC110, and hTFIIIC63 subunits of TFIIIC, the hTFIIIB90 subunit of TFIIIB, and the human RPC39 (hRPC39) and hRPC62 subunits of an initiation-specific subcomplex of RNA polymerase III. These interactions may facilitate both TFIIIB and RNA polymerase III recruitment to the preinitiation complex by TFIIIC. We show that hTFIIIC90 has an intrinsic histone acetyltransferase activity with a substrate specificity for histone H3.
ACKNOWLEDGMENTS
Y.-J.H. and T.K.K. contributed equally to this work.
We thank A. J. Berk for an hTFIIIC220 plasmid, Y. Nakatani for the p300 HAT domain clone and H3 N-terminal peptides, M. Guermah for TAFII80 baculoviruses and a TAFII100 plasmid, S. Malik for materials and technical advice on the transcription of RNA polymerase II templates, V. Palhan for a TAFII80 protein, and M. Teichmann for critical comments.
This work was supported by a grant (CA42567) from the National Institutes of Health to R.G.R.